Composite

Part:BBa_K1935016

Designed by: Jean DESCARPENTRIE   Group: iGEM16_Bordeaux   (2016-10-13)


White Collar 2 under the control of pU6 promoter in C.elegans (pU6::WC2)

This construction was made to express White Collar 1 in Caenorhabditis elegans and activate pFRQ under blue light exposure (450 nm).


Usage and Biology

See BBa_K1935006 for more details about pU6 "regulatory sequence of C.elegans".
See BBa_K1935005 for more details about White collar-2 (WC-2) "photoreceptor in Neurospora tetrasperma".


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 188
    Illegal BamHI site found at 224
    Illegal BamHI site found at 998
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1414
    Illegal NgoMIV site found at 1473
    Illegal AgeI site found at 1020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 565
    Illegal BsaI.rc site found at 933
    Illegal SapI.rc site found at 815


[edit]
Categories
Parameters
None